Rapid and specific DNA detection by magnetic field-enhanced agglutination assay

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Latex Agglutination as a Rapid Detection Assay for Spiroplasma citri

Antiserum production. Antiserum was Fletcher, J., and Slack, S. A. 1986. Latex agglutination as a rapid detection assay for Spiroplasma produced in New Zealand white ra 3 citri. Plant Disease 70:754-756. against Illinois horseradish isolates and BR6 as described (5). Antiserum titer The latex agglutination test (LAT) was effective in detecting the wall-less mollicute Spiroplasma was 1: 16,000 b...

متن کامل

Rapid detection of hepatitis B surface antigen by red cell agglutination assay.

Hepatitis B virus is an important worldwide public health problem especially in areas where the prevalence are high, such as China, Southeast Asia, tropical Africa. In Thailand, the carrier rate is about 5-10% of the total population (Kamolrata­ nakul et ai, 1985; Poovorawan et ai, 1989). The detection of hepatitis B surface antigen (HBsAg) is one of the indicators of the hepatitis B infectious...

متن کامل

Potential Use of DNA Aptamer-Magnetic Bead Separation-PCR Assay for Salmonella Detection in Food

Background: Salmonella is one of the most common food-borne pathogens that can cause illness. In this study, the sensitivity and the specificity of Aptamer-Magnetic bead Separation-Polymerase Chain Reaction (AMS-PCR) method were determined for Salmonella spp. detection. Methods: Different concentrations of Salmonella enterica were mixed with streptavidin-magnetic beads coated with biotinylated...

متن کامل

rapid and specific detection of salmonella typhimurium by pcr-elisa

objectives: salmonella typhimurium is important food-borne pathogen responsible for gastroenteritis. in this work a polymerase chain reaction based enzyme linked immunosorbent assay (pcr-elisa) was developed to identify salmonella typhimurium. materials and methods: the rfb gene which is responsible for biosynthesis of the salmonella o-antigenic lipopolysaccharide was selected as the target s...

متن کامل

A simple bridging flocculation assay for rapid, sensitive and stringent detection of gene specific DNA methylation

The challenge of bringing DNA methylation biomarkers into clinic is the lack of simple methodologies as most current assays have been developed for research purposes. To address the limitations of current methods, we describe herein a novel methyl-protein domain (MBD) enrichment protocol for simple yet rapid and highly stringent selection of highly methylated DNA from limiting input samples. We...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Talanta

سال: 2020

ISSN: 0039-9140

DOI: 10.1016/j.talanta.2020.121344